Extended Data Fig. 8: Synthesis and characterization of quadruply O-GlcNAc modified HSP27.
From: O-GlcNAc modification of small heat shock proteins enhances their anti-amyloid chaperone activity
Unmodified and differentially O-GlcNAcylated versions of HSP27 were retrosynthetically deconstructed into a recombinant protein thioester and peptides prepared by solid phase peptide synthesis. Analytical RP-HPLC traces and MALDI-TOF-MS of the indicated synthetic proteins.
