Extended Data Fig. 10: O-GlcNAc does not improve the chaperone activity of HSP27 against amorphous aggregation proteins.
From: O-GlcNAc modification of small heat shock proteins enhances their anti-amyloid chaperone activity
Citrate synthase (2 μM) in the presence or absence of the indicated HSP27 proteins (0.45 μM) were incubated at 45 °C while measuring the absorbance at 400 nm. Onset-times were obtained by measuring the time required for fluorescence to reach 3-times the initial reading. Onset-time results are mean ±SEM of n=3 independent experiments. Statistical significance was determined using a one-way ANOVA test followed by Tukey’s test.
