Fig. 2: Co-binding-mediated proximity capture of a G4 binding protein in vitro.
From: Chemical profiling of DNA G-quadruplex-interacting proteins in live cells
a, Chemical structures of G4-ligand probes photoPDS-1 (1), photoPDS-2 (2) and the control probe 3. b, Thermal melting shifts of G4 Kit1 (left) and dsDNA (right) induced by increasing concentrations of 1, 2 and 3. The increase in melting temperature (ΔTm) was measured by a fluorescence resonance energy transfer melting assay. The mean is from two independent experiments (n = 2). c, Fluorescence quenching induced by increasing the concentrations of probes 1, 2 and 3 bound to different G4 structures (G4 Myc, G4 Kit1 and G4 Telo) and dsDNA. The apparent Kd values are shown. Mean and error (± standard deviation (s.d.)) are from four independent experiments (n = 4). d, Schematic representation of the co-binding-mediated proximity capture of BG4 in vitro. e, Gel scans (probe, 10 μM) showing fluorescence images of co-binding-mediated proximity labelling of BG4 (molecular mass ~31 kDa) by 1, 2 and 3. Representative images from three independent experiments with similar results are shown.
