Extended Data Fig. 3: Dynamics in the visible range.
From: Ultrafast proton-coupled isomerization in the phototransformation of phytochrome
Dynamics in the visible range. (a) Decay associated spectra of Agp2-WT at pH 7.5 excited at 740 nm (40 nm FWHM, < 40 fs time-resolution). Left panel shows the isotropic decay associated spectra. The right panels show the same components polarization resolved. Thicker lines present parallel polarization of pump and probe, thinner lines the perpendicular polarized case. (b) Transients of the same dataset; data (colored) and resulting model (black) for selected frequencies. Left panel shows isotropic data and simulations, while the right panel shows the polarization resolved data. Thick lines are for parallel polarization, thin lines for perpendicular polarization. Used decay constants are 50 fs, 150 fs, 1.5 ps, 4 ps and a constant component. For the very early delay times small deviations between modelled transients and data are visible. Introducing an additional time constant around 100 fs would have resulted in reduced deviations, but also in an ill-conditioned model. (c) Comparison of the solvent signal (red) with the sample signal (blue) at different spectral positions and different polarization directions with respect to pump pulse polarization.
