Extended Data Fig. 2: Evaluation of RNA substrate cleavage site preferences by the FR6_1 XNAzyme. | Nature Chemistry

Extended Data Fig. 2: Evaluation of RNA substrate cleavage site preferences by the FR6_1 XNAzyme.

From: A modular XNAzyme cleaves long, structured RNAs under physiological conditions and enables allele-specific gene silencing

Extended Data Fig. 2

(a) Sequence of “FR6_1_NucSR”, a variant of XNAzyme FR6_1 retargeted to cleave RNA substrate “NucSR”(see reference (56)) by changing FANA residues shown in brown. (b) Urea-PAGE gels showing reactions of FR6_1_NucSR (5 uM) with all possible variants of RNA substrate NucSR (1 uM) at positions 8, 9 and 10 (shown in red in (a))(24 h, 17 °C, 10 mM Mg2+).

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