Fig. 1: Ribosomal incorporation of cγAAs into a macrocyclic peptide library. | Nature Chemistry

Fig. 1: Ribosomal incorporation of cγAAs into a macrocyclic peptide library.

From: In vitro selection of macrocyclic peptide inhibitors containing cyclic γ2,4-amino acids targeting the SARS-CoV-2 main protease

Fig. 1

a, Structures of the cγAAs and cβAAs used in this study. b, The reprogrammed codon table that contains ClAcy at the initiator AUG codon, and c, γ1, γ2, β1 and β2 at the elongator AUG, GAG, GUG, GUU and UGU codons, respectively. NNA codons are omitted because they were not used in the mRNA library. c, Construction of the random mRNA library and the corresponding peptide library. Peptides spontaneously macrocyclized between ClAcy and c via a thioether bond. The mRNA and peptide were covalently linked via a puromycin linker.

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