Extended Data Fig. 1: RaPID selection against Mpro using a macrocyclic peptide library containing cγAA. | Nature Chemistry

Extended Data Fig. 1: RaPID selection against Mpro using a macrocyclic peptide library containing cγAA.

From: In vitro selection of macrocyclic peptide inhibitors containing cyclic γ2,4-amino acids targeting the SARS-CoV-2 main protease

Extended Data Fig. 1

a, Schematic depiction of RaPID display. 1) Puromycin linker ligation to the 3′-end of the mRNA library. 2) Translation of peptides using the reprogrammed genetic code, followed by spontaneous macrocyclization of peptide via a thioether bond. 3) Reverse transcription of mRNA into cDNA. 4) Binding selection of peptides against Mpro immobilized on magnetic beads. 5) Recovery of the bound fraction and amplification of cDNA by PCR. 6) Transcription of cDNA library into mRNA library. 7) Deep sequencing analysis of cDNA library. b, Recovery rate of cDNA after the binding selection at each round. Red and blue bars indicate the recovery rate of Mpro binders and magnetic beads binders, respectively. Bead-binder selection was not performed in the first round.

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