Extended Data Fig. 6: Plate reader analysis of sfGFP expression in DH10B E. coli with n = 4 replicates. | Nature Chemistry

Extended Data Fig. 6: Plate reader analysis of sfGFP expression in DH10B E. coli with n = 4 replicates.

From: Expanding the substrate scope of pyrrolysyl-transfer RNA synthetase enzymes to include non-α-amino acids in vitro and in vivo

Extended Data Fig. 6: Plate reader analysis of sfGFP expression in DH10B E. coli with n = 4 replicates.The alternative text for this image may have been generated using AI.

Emission at 528 nm after 24 h sfGFP expression in E. coli DH10B cells harboring pEVOL or pMega plasmids encoding a, MaPylRS and Ma-tRNAPyl in the presence of 0 or 1 mM BocK (1) or α-OH BocK (2) or b, MaFRSA and Ma-tRNAPyl in the presence of 0 or 1 mM m-CF3-L-phenylalanine (20) or α-OH m-CF3- phenylalanine (21).

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