Extended Data Fig. 2: Resolution of the 50S subunit in the Met-Met ribosome structure. | Nature Chemistry

Extended Data Fig. 2: Resolution of the 50S subunit in the Met-Met ribosome structure.

From: Atomistic simulations of the Escherichia coli ribosome provide selection criteria for translationally active substrates

Extended Data Fig. 2

a, Local resolution of the 50S-focused cryo-EM map; b, FSC curves indicating resolution of the 50S subunit. Half-map FSC (black) is at 2.10 Å at the gold-standard cutoff value 0.143; map-vs.-model FSC (gray) is at 2.06 Å at the 0.5 cutoff. Only the large subunit was masked for the half-map FSC, and c, representative micrograph showing individual ribosome particles. The micrograph was low-pass filtered to 20 Å for easier visualization of particles.

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