Extended Data Fig. 5: Comparison of the PP vs PDP TR-SFX light induced differences in the rsKiiro chromophore region.
From: Optical control of ultrafast structural dynamics in a fluorescent protein
a. Difference maps for the chromophore region, hydrogen bonded water and His194 for the femtosecond time bins for PP (Left) and PDP (right) data. The coordinates for ground state (yellow carbons) and the refined coordinates for light data after partiality refinement (grey carbons) are shown with electron density contoured at +3σ rms (blue) and -3σ rms (red).A 1.5 Å resolution cutoff was used. b. Same representation for the merged 0-1 ps PP delays with key difference peaks numbered. c. Difference density peak values at the locations shown in panel b. Plotted as a solid line (with dots) and a dashed line (with diamonds) for the PP and PDP respectively. Shown in blue for positive values of density, and in red for negative. The time dependence of difference features elsewhere in the protein (Fig. 1) showed a similar multi-mode character, but with different dependence for most features.
