Extended Data Fig. 6: Comparison of rsKiiro TR-SFX Pump-Probe vs Pump-Dump-Probe difference maps.
From: Optical control of ultrafast structural dynamics in a fluorescent protein
a. Q-weighted difference electron density for each bin of the Pump-Probe (400 nm) on the left and of the Pump-Dump-Probe (400-515 nm) on the right. The secondary structure (green) of the ground state coordinates (yellow) are shown with electron density contoured at +3σ rms (blue) and -3σ rms (red). b. Integrated electron difference density above 3σ level within 2 Å of each sequential atom in the protein chain (in residue number order) for Pump-Probe data 3375set. Plotted for each time bin is the positive (blue) and negative (red) electron density, which are normalized with respect to all other time bins. Shown at the bottom is a greyscale bar indicating the distance from the center of the chromophore to the atom number65 c. and d. The same analysis for the Pump-Dump-Probe case.
