Extended Data Fig. 7: Extrapolated maps and difference maps of merged 0-1 ps TR-SFX of rsKiiro with refined coordinates.
From: Optical control of ultrafast structural dynamics in a fluorescent protein
a. Pump-probe illumination scheme. Map showing extrapolated F0 electron density for NEXT = 13.6, contoured at 1.5 rms (yellow mesh) with the ground state (yellow) and extrapolated (grey) refined coordinates. Q-weighted difference electron density between the dark and 400 nm 0-1 ps is shown at 3 rms level with positive and negative signals in blue and red respectively. There is a 180° rotation between the left and right representations. b. Pump-Dump-Probe illumination scheme, with same representation except a value of NEXT = 9 was chosen as it was closest to R-factor minimization occupancies of 21 % using the approximation: PT ~ 200/ NEXT where PT is population transfer.
