Extended Data Fig. 4: Femtosecond time resolved Infrared and FTIR spectroscopy of rsKiiro.
From: Optical control of ultrafast structural dynamics in a fluorescent protein
a. steady state FTIR difference spectra of the off minus the on state of rsKiiro. Conversion to the off state was achieved by illuminating the sample with a 500 nm LED. b. TR-IR pump-probe measurements of the off state when pumped with 400 nm 100 fs pulses. The fitted EADS spectra are shown (top) and selected spectra at various pump-probe delays (bottom). Greyed out bands correspond to the labelled assignments. Note: For the on state there is overlap of the C = O and Arg66 asym CN3H5+ in the 1645-1670 cm-1 region. c. Dronpa and rsKiiro on and off state FTIR peak assignments for 2H and 1H buffers. Dronpa assignments made by Warren et al. All values in units of cm-1.
