Fig. 3: Condensation of B-LCD accelerates amyloid formation.
a, Protein concentration inside (CD, blue cross) and outside (CS, blue circle) the condensates, measured as in Fig. 1. Asterisks indicate initial protein concentration in kinetic experiments in b and c: 1 µM (yellow), 10 µM (green), 15 µM (red), 20 µM (blue) and 30 µM (black). Error bars represent the standard deviation of the average protein concentration inside and outside the droplets, obtained by measuring three different droplets (for CD) and three independent samples (for CS) at each C0. The blue dashed line represents a guide to the eyes and indicates approximated upper and lower phase boundaries considering the total concentration of salt as a proxy for the intermolecular interaction parameter. b, Average ThT fluorescence intensity value of three replicates of 1 µM and 10 µM B-LCD solutions after 7 days incubation respectively below and above the critical concentration of LLPS (Cc = 2.5 µM). Error bars represent the standard deviation of the three replicates. c, Normalized ThT kinetic profiles corresponding to the initial protein concentrations C0 in a. The inset shows the average lag times t0.1 at various initial protein concentrations C0. Error bars represent standard deviation of three independent samples. d, Droplets formed at low and high initial B-LCD concentration exhibit similar internal protein concentration (CD) but increase in number when the initial protein concentration is increased.
