Extended Data Fig. 7: qPCR independently validates the transcriptome interactions of Hydroxychloroquine (HCQ) and Dasatinib (Das) at several transcriptome binding sites in HEK293 cells.
From: Pervasive transcriptome interactions of protein-targeted drugs
a, Workflow showing the strategies of validating competable 2´-OH acylation sites with qPCR (Top panel). b-c, qPCR validated and quantified the relative level of 2´-OH acylation at the drug-binding loci of HCQ (b) and Das (c). Data represent mean ± s.e.m., n = 3 biologically independent experiments. Statistical significance was calculated with two-tailed Student’s t-tests: *P < 0.05, **P < 0.01, ****P < 0.0001. P values are <0.0001 (ANKRD28), 0.0198 (ATP4A), 0.0011 (ATP5PB), and 0.0254 (FAM168A), respectively.
