Extended Data Fig. 2: RNA-seq determines how excess unmodified drug influences transcripts abundance in HEK293 cells.
From: Pervasive transcriptome interactions of protein-targeted drugs
a, Workflow of RNA-seq with HEK293 cells treated with unmodified drugs or DMSO. b-d, Volcano plot showing effects of unmodified Lev (b), HCQ (c), and Das (d) on transcript abundance. X-axis: log-transformed ratio of transcript abundance in the presence over the absence of unmodified drug. Y-axis: the negative value of log-transformed P-value calculated with DESeq2 using a two-tailed Wald test. Fold-change=transcript abudance (drug-treated)/transcript abudance (DMSO).
