Extended Data Fig. 3: Additional information from polymer model simulations.
From: Determinants that enable disordered protein assembly into discrete condensed phases
a. Density profiles of polymer B and EI plot for λAB = λBB case. b. Colormap of EI from CG scanning simulation with varying λAB and λBB. c. Plot showing variation of EI as a function of λAB at λBB = 0 highlights the complex coacervate case. d. Plot showing variation of EI as a function of λBB/λAB at different constant λAB values. Maximum enrichment is observed when ratio of λBB/λAB is close to 1. e. Density profiles of polymer A and B corresponding to the simulation representations in Fig. 3e. f. Density profile from single component CG slab for varying interaction strength. For interaction strength for 0.75 or above, chains start to condense. g. Simultaneous changes in homotypic and heterotypic interaction parameters can yield experimentally observed plateauing in EI. Plot shows variation of EI (right) along an arbitrarily defined change in λAB and λBB values (blue line shown in heat map (left)). Right plot shows plateauing of EI with increasing λBB (bottom x-axis) and λAB (upper x-axis). For +20Tyr and +30Tyr, increasing tyrosine will increase both homotypic and heterotypic interactions and scanning simulation shows one can obtain similar EI values as the FUS LC wildtype. For clarity, we only show one representative combination of λAB and λBB, but there are several other possible combinations that can yield similar EI values as the FUS LC wildtype. Data relevant to Fig. 3.
