Extended Data Fig. 1: hnRNPA1A amyloid fibrils are oriented parallel to the interface of the condensates.
From: RNA modulates hnRNPA1A amyloid formation mediated by biomolecular condensates
To further prove the orientation of fibrils at the interface of the condensates, we applied Fluorescence Detected Linear Dichroism (FDLD) in combination with ThT staining to analyse amyloid fibrils formed at later incubation times (24-48 h). From the images, it is possible to observe that ThT molecules bound to horizontally oriented fibrils are preferentially excited by horizontally polarized light (color-coded in blue in the FDLD image, see Material and Methods). In contrast, ThT molecules bound to vertically oriented fibrils are preferentially excited by vertically polarized light (color-coded in yellow in the FDLD image). This experiment was performed in technical duplicates for one single protein batch.
