Extended Data Fig. 2: Measurement of monomer conversion using Fluorescence Correlation Spectroscopy (FCS).
From: RNA modulates hnRNPA1A amyloid formation mediated by biomolecular condensates
Representative auto-correlation curves acquired on atto647N hnRNPA1A after 72h of incubation with (A) 0 ng/ul polyU (B) 50 ng/ul polyU and (C) 500 ng/ul polyU. The curves were fitted with a model assuming a single diffusing component. Data points represent the mean and error bars standard deviation of the measurement. The fitting residuals are showed below each graph. The amplitude decrease of the auto-correlation curve with increasing polyU concentration indicates that RNA contributes to protein solubility. hnRNPA1A concentration calculated in each condition was 1.68 ± 0.51 uM in absence of polyU, 2.5 ± 0.18 uM with 50 ng/ul uM polyU and 4.68 ± 0.20 uM.
