Extended Data Fig. 6: Reconstructed ESI-MS spectra for more timepoints from the single-domain antibody heterodimer labelling experiment described in Fig. 5c.
From: Repurposing a plant peptide cyclase for targeted lysine acylation
The 4 h timepoint data is also shown in Fig. 5c. Timepoints for a control experiment on a protein where the Lys in the KL-tag was mutated to Ala are also shown. Reactions were run in 100 mM HEPES buffer, pH 8, containing 50 µM protein, 750 µM biotin-RNGLH or 1 mM TAMRA-RNGLH, 1 mM NiSO4, and 1 µM OaAEP1 for the indicated timepoints at 25 °C. Mass shifts: +biotin-RN, +497 Da (calc.: +497 Da); +TAMRA-RN, +682 Da (calc.: +683 Da).
