Extended Data Fig. 7: Reconstructed ESI-MS spectra for timepoints from the CTC-445.2d labelling experiment described in Fig. 5d.
From: Repurposing a plant peptide cyclase for targeted lysine acylation
Shown is labelling with biotin-RNGLH or TAMRA-RNGLH. Reactions were run in 100 mM HEPES buffer, pH 8, containing 50 µM protein (either KL-tagged or a variant where the Lys-Leu sequence was mutated to Ala-Leu), 1 mM TAMRA-RNGLH or biotin-RNGLH, 1 mM NiSO4, and 1 µM OaAEP1 for the indicated timepoints at 25 °C. Mass shifts: +biotin-RN, +497 Da (calc.: +497 Da); +TAMRA-RN, +682 Da (calc.: +683 Da).
