Extended Data Fig. 3: Isopeptide ligation on a Lys-Leu peptide in the presence of increasing Lys-Ala peptide concentrations.
From: Repurposing a plant peptide cyclase for targeted lysine acylation
Reactions containing 100 µM of a model NGLH-containing acyl donor peptide (Ac-RWRGWRNGLH), 200 µM of a Lys-Leu-containing peptide, 0-2,000 µM of a Lys-Ala-containing peptide (as indicated), 200 µM Ni2+, and 1 µM OaAEP1 were run in 100 mM HEPES buffer, pH 8.5, for 3 h at 25 °C. Conversion (%) of the NGLH-containing substrate to one or the other isopeptide-linked products was determined via RP-HPLC (n = 3 independent experiments).
