Extended Data Fig. 4: The conversion rates in the coupling reactions between IPA (S2) or HPPA (S3) and aliphatic α-keto acids catalyzed by CsmA, BbmA and the double mutants CsmAS110L/T278W, BbmAL112S/W288T. | Nature Chemistry

Extended Data Fig. 4: The conversion rates in the coupling reactions between IPA (S2) or HPPA (S3) and aliphatic α-keto acids catalyzed by CsmA, BbmA and the double mutants CsmAS110L/T278W, BbmAL112S/W288T.

From: Structural insights into two thiamine diphosphate-dependent enzymes and their synthetic applications in carbon–carbon linkage reactions

Extended Data Fig. 4

(a)–(g), The total conversion rates of the two NaBH4-reduction products during the coupling reactions between IPA (S2) and PVA (S1, a), MOBA (S4, b), OPTA (S12, c), 3-MOVA (S13, d), OHA (S15, e), CPOA (S16, f), or CBOA (S17, g), respectively. (h)–(o), The total conversion rates of the two NaBH4-reduction products during the coupling reactions between HPPA (S3) and PVA (S1, h), MOBA (S4, i), OPTA (S12, j), 3-MOVA (S13, k), 4-MOVA (S14, l), OHA (S15, m), CPOA (S16, n), or CBOA (S17, o), respectively. All chromatograms are shown in the Supplementary Information (Supplementary Figs. 7 and 8). The conversion rates in percentile were determined from three original independent replicates of reactions (shown as dots) and the error bars were generated as mean values +/− SD (standard deviations) from the three independent replicates.

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