Extended Data Fig. 5: Fluorescence recovery after photobleaching (FRAP) reveals dynamical arrest of the RNA component, TERRA, but not RGG and d(T)40 with condensate aging. | Nature Chemistry

Extended Data Fig. 5: Fluorescence recovery after photobleaching (FRAP) reveals dynamical arrest of the RNA component, TERRA, but not RGG and d(T)40 with condensate aging.

From: Homotypic RNA clustering accompanies a liquid-to-solid transition inside the core of multi-component biomolecular condensates

Extended Data Fig. 5: Fluorescence recovery after photobleaching (FRAP) reveals dynamical arrest of the RNA component, TERRA, but not RGG and d(T)40 with condensate aging.The alternative text for this image may have been generated using AI.

(a) Schematic of FRAP assay in condensate. (b) Fluorescence images of (TERRA)4 containing RGG-d(T)40 condensates at pre-bleach, bleach, and post-bleach steps of FRAP experiments corresponding to Fig. 5c and Supplementary Videos 11–16. Shaded regions in each plot signify the standard error. The composition of the (TERRA)4 containing RGG-d(T)40 condensate system used here is 1 mg/ml (TERRA)4 (corresponds to 127 μM), 5 mg/ml RGG, and 1.5 mg/ml d(T)40 in a buffer containing 25 mM Tris-HCl (pH 7.5), 25 mM NaCl, and 20 mM DTT. The concentration of labeled components is 250 nM. Each experiment was independently repeated three times.

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