Extended Data Fig. 6: Cluster size measurements in quaternary condensate systems show the efficacy of ASO or G3BP1 in buffering intra-condensate RNA clustering. | Nature Chemistry

Extended Data Fig. 6: Cluster size measurements in quaternary condensate systems show the efficacy of ASO or G3BP1 in buffering intra-condensate RNA clustering.

From: Homotypic RNA clustering accompanies a liquid-to-solid transition inside the core of multi-component biomolecular condensates

Extended Data Fig. 6: Cluster size measurements in quaternary condensate systems show the efficacy of ASO or G3BP1 in buffering intra-condensate RNA clustering.The alternative text for this image may have been generated using AI.

The cluster size plots from SAC at distinct sample ages for (a) ASO-treated (TERRA)10 containing ternary condensate system, (b) G3BP1-treated (TERRA)10 containing ternary condensate system, (c) G3BP1-treated r(CAG)31 containing ternary condensate system, and (d) G3BP1-treated r(CUG)47 containing ternary condensate system. The detection limit of SAC is demarcated (see Methods for further details). All box plot elements are defined similarly to Fig. 2d. The concentrations of the ASO and G3BP1 are 1 mg/ml and 10 μM, respectively. The composition of the ternary condensate system used in these experiments is 1 mg/ml RNA [0.45 mg/ml in the case of r(CUG)47; (TERRA)10, 101 μΜ, r(CAG)31, 33 μM; r(CUG)47, 10 μM], 5 mg/ml RGG, and 1.5 mg/ml d(T)40. Buffer composition for all experiments is 25 mM Tris-HCl (pH 7.5), 25 mM NaCl, and 20 mM DTT. The concentration range of labeled components is 250 nM to 500 nM. The sample size for each condition is 10 condensates, representative of three biological replicates.

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