Extended Data Fig. 8: G3BP1 buffers intra-condensate RNA clustering. | Nature Chemistry

Extended Data Fig. 8: G3BP1 buffers intra-condensate RNA clustering.

From: Homotypic RNA clustering accompanies a liquid-to-solid transition inside the core of multi-component biomolecular condensates

Extended Data Fig. 8: G3BP1 buffers intra-condensate RNA clustering.The alternative text for this image may have been generated using AI.

(top) Fluorescence images utilizing FAM-(TERRA)4 and Cy5-d(T)40 of RGG-d(T)40 condensates containing (TERRA)10 along with G3BP1. (bottom) Cluster sizes derived from SAC are reported. The detection limit of SAC is demarcated. These observations correspond to data shown in Fig. 6. The composition of the condensate system used here is 10 μM G3BP1, 1 mg/ml RNA (corresponds to 50.7 μM), 5 mg/ml RGG, and 1.5 mg/ml d(T)40 in a buffer containing 25 mM Tris-HCl (pH 7.5), 25 mM NaCl, and 20 mM DTT. The concentration of labeled components is 250 nM. The sample size for each condition is 10 condensates, representative of three biological replicates.

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