Extended Data Fig. 1: TERRA clustering propensity is independent of the bulk RNA concentration. | Nature Chemistry

Extended Data Fig. 1: TERRA clustering propensity is independent of the bulk RNA concentration.

From: Homotypic RNA clustering accompanies a liquid-to-solid transition inside the core of multi-component biomolecular condensates

Extended Data Fig. 1: TERRA clustering propensity is independent of the bulk RNA concentration.The alternative text for this image may have been generated using AI.

Effect of titration of bulk RNA concentration in (a) (TERRA)10 and (b) (TERRA)4 containing ternary RGG-d(T)40 condensate systems on RNA cluster formation upon aging. The condensates and RNA clusters were visualized using FAM-labeled (TERRA)4. The composition of the condensate systems used here is 5 mg/ml RGG, variable d(T)40, and variable (TERRA)10 concentrations (as indicated in the figure panels), with the total nucleic acid concentration kept constant at 2.5 mg/ml. TERRA concentration (in mg/ml and molarity) used in each sample is indicated in each panel of the figure. The buffer contained 25 mM Tris-HCl (pH 7.5), 25 mM NaCl, and 20 mM DTT. The concentration of FAM labeled (TERRA)4 is 250 nM. Each measurement was independently repeated three times.

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