Fig. 3: Controlling cell-free protein synthesis with an alternating magnetic field.
From: Magnetic activation of spherical nucleic acids enables the remote control of synthetic cells
a, Schematic detailing the release of the T7 promoter top sequence from the SNA surface as a result of inductive heating and exposure to an AMF, and the subsequent hybridization of the promoter sequence to the single-stranded T7 promoter of the inactive template, restoring the dsDNA T7 promoter and activating expression of the encoded fluorescent mNG protein. Note: promoter (23 bp) and gene (∼700 bp) length are not to scale. T, temperature. b, Cell-free protein synthesis of mNG in the presence of magnetically activated SNAs, with and without exposure to an AMF. Reactions that were not exposed to an AMF expressed minimal mNG, and their fluorescence intensity was comparable to the inactive DNA template only. Reactions exposed to an AMF expressed mNG and their fluorescence was consistent with reactions containing the full mNG template (dsDNA T7 promoter region present). Representative of n = 3 biological replicates. Data are presented as mean values with error bars representing the s.d. **P < 0.02 (P = 0.016); NS, not significant (P = 0.30) from one-tailed t-tests.
