Extended Data Fig. 3: Stopped-flow analysis of O2 activation by PolF.
From: Azetidine amino acid biosynthesis by non-haem iron-dependent enzymes
(a) Absorption spectra acquired after a rapid mixing at 5 °C of an anoxic solution of 0.3 mM PolF and 0.6 mM Fe(II) with an equal volume of O2-saturated buffer. (b) Absorption kinetic traces monitored at 614 nm. An anoxic solution of PolF (0.3 mM), Fe(II) (0.6 mM, 2 molar equivalent), and substrate (1 mM l-Ile or 1 mM l-Val or no substrate) was mixed with an equal volume of O2-saturated buffer. (c and d) Stopped-flow analysis of O2 activation by PolF with 3,4-dhVal. Absorption spectra (c) and absorption kinetic traces monitored at 614 nm (d) acquired after a rapid mixing at 5 °C of an anoxic solution of 0.3 mM PolF and 0.6 mM Fe(II), 1 mM 3,4-dhVal with an equal volume of O2-saturated buffer. Source data are provided.
