A chemical photocatalytic sequencing system, CAT-seq, enables high-resolution profiling of mitochondrial RNA in living cells without requiring genetic manipulations. Integrating CAT-seq with a thio-quinone methide probe for protein labelling resulted in CAT-ortho, a platform for simultaneous RNA–protein labelling. CAT-ortho revealed distinct metabolic remodelling mechanisms in macrophages and primary T cells.
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References
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This is a summary of: Bi, Y. et al. Photocatalytic labelling-enabled subcellular-resolved RNA profiling and synchronous multi-omics investigation. Nat. Chem. https://doi.org/10.1038/s41557-025-01946-1 (2025).
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Simultaneous subcellular RNA–protein profiling with photocatalytic labelling. Nat. Chem. 17, 1817–1818 (2025). https://doi.org/10.1038/s41557-025-01963-0
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DOI: https://doi.org/10.1038/s41557-025-01963-0
