Extended Data Fig. 2: G4L-PROTACs induce degradation of exogenous G-quadruplex binding proteins. | Nature Chemistry

Extended Data Fig. 2: G4L-PROTACs induce degradation of exogenous G-quadruplex binding proteins.

From: Degradation of G-quadruplex-binding proteins in chromatin using G4-ligand-based proteolysis-targeting chimeras

Extended Data Fig. 2: G4L-PROTACs induce degradation of exogenous G-quadruplex binding proteins.

a, Flow cytometry analysis of U2OS cells expressing SG4-GFP, a G4-specific nanobody fused to GFP, following treatment with different concentration of G4L-PROTAC2 (1–5: 10 μM, 5 μM, 2 μM, 200 nM, 20 nM treatments respectively). Histograms (top left) and dot plots (right) show a dose-dependent reduction in GFP fluorescence, indicating degradation of the SG4-GFP reporter. A schematic of the SG4-GFP construct and its G4 binding is shown bottom left. b, Flow cytometry analysis of U2OS cells expressing G4P-RFP, a G4-binding protein fused to RFP, after treatment with different concentration of G4L-PROTAC2 (1–5: 10 μM, 5 μM, 2 μM, 200 nM, 20 nM treatments respectively). Reduced RFP signal (top left histogram) and corresponding dot plots (right) confirm target degradation. Schematic illustration of G4P-RFP and its G4 interaction is shown bottom left. In both panels, control represents doxycycline untreated cells.

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