Extended Data Fig. 7: Characterization of five FABLE Designs.
From: Emergence of specific binding and catalysis from a designed generalist binding protein
Left column: Fluorescence of Cou485 at two fixed concentrations of 3 μM and 6 μM were measured in the presence of increasing amounts of each protein. The dissociation constant was obtained by globally fitting a single-site binding model to data, as described at Supplementary Methods. The error bars represent standard deviations of three independent measurements. Middle Column: circular dichroism spectra shows that all the designs are helical proteins. Right column: temperature-dependent circular dichroism signals measured at 222 nm show that all designs are thermostable. The design 1 was designated as FABLE.
