Extended Data Fig. 4: PAR-activation shifts β₁-class integrins into high-affinity conformation.

Suspended parental fibroblasts were incubated first with 0.1 mM control, PAR1- or PAR2-activating peptide, or 1 mM Mn²⁺ for 20 min, then incubated with monoclonal antibodies either detecting the high-affinity conformation of β₁-class integrins (9EG7) or detecting all β₁-class integrins (KMI6). Subsequently, fibroblasts were incubated with the same secondary antibody and analyzed by flow cytometry. Each dot represents the median of one flow cytometry measurement (n(exp) = 12 per condition), normalized to the median fluorescence intensity of fibroblasts in the absence of peptide and Mn²⁺. Black bars indicate the mean of all flow cytometry experiments. Two-tailed Mann-Whitney tests were used to determine P-values between activating peptide or Mn²⁺ and control peptide.