Extended Data Fig. 7: Representative morphologies of pKO-β1 and pKO-αV fibroblasts adhering to FNIII7-10. | Nature Materials

Extended Data Fig. 7: Representative morphologies of pKO-β1 and pKO-αV fibroblasts adhering to FNIII7-10.

From: Protease-activated receptor signalling initiates α5β1-integrin-mediated adhesion in non-haematopoietic cells

Extended Data Fig. 7: Representative morphologies of pKO-β1 and pKO-αV fibroblasts adhering to FNIII7-10.The alternative text for this image may have been generated using AI.

Serum-starved pKO-β1 and pKO-αV fibroblasts were allowed to adhere for the indicated time on FNIII7-10-coated dishes at 37 °C, washed and fixed. The experiment was done in presence of 0.1 mM control or PAR2-activating peptide. Representative cells are exemplified. Scale bars, 25 µm. For adhesome proteomics we had to wait 10 min until pKO-β1 fibroblasts and 15 min until pKO-αV fibroblasts immobilized on the dishes before crosslinking. Representative pKO-β1 and pKO-αV fibroblast morphologies after different adhesion times. The first time frames correspond to the time point fibroblasts were used for adhesome proteomics analysis. At least 6 fibroblasts were analyzed per experimental condition.

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