Extended Data Fig. 2: PAR2-activating peptide and PAR2 agonist increase adhesion forces of parental fibroblasts to similar levels.
a, Adhesion force of parental, pKO-β1 and pKO-αV fibroblasts, adhering to FNIII7-10-coated (dark colors, black bars) or FNIII7-10∆RGD-coated (light colors, grey bars) supports. Experiments were conducted in the absence and presence of 1 µM control peptide. The control peptide did not influence the adhesion force to FNIII7-10 or FNIII7-10∆RGD measured after 60 s contact time for any fibroblast line. b, Adhesion force of parental fibroblasts adhering to FNIII7-10-coated (dark colors, black bars) or FNIII7-10∆RGD-coated (light colors, grey bars) supports after incubation with different concentrations (as indicated) of control, PAR1-activating, or PAR2-activating peptide. The experiments, which measure the adhesion force after 60 s contact time to FNIII7-10-coated supports, validate that the peptide concentrations used in our experiments are sufficient to elicit a full cellular response. Additionally, the experiments show that the small molecule PAR2 agonist AC264613 increases fibroblast adhesion to FNIII7-10 similar to as detected for the PAR2-activating peptide. Top or lower n(cells) give the number fibroblasts tested on FNIII7-10-coated or FNIII7-10∆RGD-coated supports, respectively. Dots represent adhesion forces of single fibroblasts and bars median values. Statistical differences analyze fibroblast adhesion to FNIII7-10-coated supports in the presence of 0.1 and 1.0 mM peptide or 25 µM PAR2 agonist using the two-tailed Mann-Whitney test. P-values are given if < 0.05.
