Extended Data Fig. 8: Influence of printing cell density on tube formation and diameter.
From: Recapitulating macro-scale tissue self-organization through organoid bioprinting
a, Representative tube printed from small intestinal organoids (that is not single cells). Top: 1 hour after printing, Middle: 72h after printing, Bottom: staining (Dapi labelling the nuclei, Actin) of the tube showing a lumen and crypt structures. Scale bars, 200 µm. b, Change of mean tube diameter over time at three different cell densities: 12,5 million (white symbol), 25 million (white and black symbol) and 50 million (black symbol) cells/ml. The experiment was repeated three times and two tubes are shown for each cell density. c, Evolution over time of one representative tube for each initial cell density from one of the three experiment is shown. Scale bars, 1000 µm.
