Extended Data Fig. 9: Effect of IMC co-culture in bioprinted intestinal tubes.
From: Recapitulating macro-scale tissue self-organization through organoid bioprinting
a, Bright-field images and corresponding fluorescent images of Lgr5-eGFP three days after printing, showing the effect of IMC co-culture on tube formation. IMCs were either deposited after printing for adherence on the well bottom and hydrogel (left), or directly suspended inside the hydrogel before and during printing (right). In all cases, a continuous lumen was formed after only one day, and the tubes comprise many Lgr5+ buds after 3 days. Images are representative of n = 3 biologically independent experiments. Scale bars, 250 µm. b, Difference in evolution of the tube diameter if co-cultured with IMCs or not. For each day, the mean diameter (dotted line) of tubes with and without myofibroblasts were compared (n=3 tubes for each day).
