Extended Data Fig. 6: Microtubule acetylation retains E-cadherin at the NC cell-cell contacts.
From: Cell clusters softening triggers collective cell migration in vivo
(a) Schematic showing a graft experiment and the plane of sectioning post graft (hm, head mesoderm; ML, mediolateral; AP, anteroposterior; DV, dorso-ventral). (b) In the upper panel, representative confocal projections of transverse cryosections showing NC from embryos grafted with WT α-tubulin-GFP or HyperAcet α-tubulin-GFP; in the lower panel, colour-coded confocal projections showing the intensity of E-cadherin; the cyan circle delimits the neural crest position (scale bar, 100 μm). (c) Chart showing the quantification of E-cadherin max intensity across NC membranes. Fluorescent intensity over a 10 μm distance is shown, middle solid lines represent median and shadow the interquartile range; (two tailed Mann-Whitney test, **** P < 0.0001, CI = 95%, ncontrol = 21, nHyperAcet = 21 junctions). (e) Lateral views of embryos hybridised with a probe against sox8, conditions as indicated (scale bar 200 μm). b,d,e, representative examples from at least 3 independent experiments.
