Extended Data Fig. 7: Targeted injections into the head mesoderm and hydrogels system validations.
From: Cell clusters softening triggers collective cell migration in vivo
(a) Schematic displaying targeted injections into the mesoderm. (b) Drawing shows the plane of cryosection (hm, head mesoderm), and confocal projections display the result of targeting nuclear RFP into the head mesoderm (red); NC nuclei are also shown (cyan) (scale bar, 150 μm). (c–e) Characterisation of the ex vivo system that reproduces the stiffness values that neural crest cells experience at non- and migratory stages. (c) Orthogonal view of a confocal projection of soft and stiff hydrogels. Images in (c) are representative examples from at least 3 independent experiments (scale bar, 50 μm). (d) Chart showing that the layer of Fibronectin has similar thickness in soft and stiff gels; spread of data is shown; data points represent the mean obtained from each gel and at least 5 measurements were taken per gel; red line represents mean, and whiskers show SD (two-tailed t-test ****P < 0.0001, CI = 95%, n = 5 gels;). (e) AFM measurements obtained from soft and stiff hydrogels; spread of data is shown; each data point represents the mean of a gel, and 64 indentations were performed per gel; red lines show mean and whiskers SD; n = 5 gels; two-tailed t-test, ****P < 0.0001, CI = 95% (scale bar, 50 μm). b, representative examples from at least 3 independent experiments.
