Extended Data Fig. 9: Piezo1 mechanosensing mediates NC microtubule acetylation.
From: Cell clusters softening triggers collective cell migration in vivo
(a) Immunofluorescence analysis showing acetylated α-Tubulin and α-Tubulin signal in NC plated in permissive substrates (nuclei is shown in magenta and NC border is depicted by cyan solid lines); treatments and channels as indicated. Images are representative examples from at least 3 independent experiments (scale bar 25μm). (b) Chart showing the normalised fluorescence intensity ratio of acetylated α-Tubulin vs α-Tubulin; spread of data is shown, red lines represent mean, whiskers represent SD (two tailed t-test ****P < 0.0001, CI = 95%, n1–3 = 37 cells). a, representative examples from at least 3 independent experiments.
