Extended Data Fig. 1: in vivo Atomic Force Microscopy experiments setup.
From: Cell clusters softening triggers collective cell migration in vivo
(a) Image of the AFM cantilever position relative to the neural crest (cyan) and head mesoderm (magenta). A heat map showing measurements acquired in an 8×8 grid with 6.25 μm x/y space resolution is shown; this grid depicts the spread of data found in the tissues we measured. All measurements were recorded by using a cantilever carrying a 10 μm diameter bead as a tip. Each data point in our charts presented in the main figures represents an embryo from which the median resulting from our 8×8 grids was calculated. (b) Representative example of a force-distance curve obtained using cantilevers coated with 10 μm beads. Mean indentation depth (δ) across all our measurements with its respective standard deviation is also shown. (c) Image of AFM cantilever position relative to the neural crest nuclei (left) and nuclei particle image velocity (PIV) displacement heat map result (right) (scale bar 50 μm). c, representative examples from at least 3 independent experiments.
