Extended Data Fig. 5: Expression of BCR pathway proteins in ABC-DLBCL organoids.
a, Left: Representative flow cytometry histograms. Right: Median fluorescent intensity (MFI) of BCL10 in human PDX cells cultured in ±CD40L-stromal cell conditions for 96 h. Two-tailed unpaired t-test (mean ± s.e.m., n = 4 for PDX#4; n = 5 for PDX#5; where each dot is a hydrogel-based organoid). b, MFI of BCL10 in human ABC-DLBCL PDX cells cultured in ±CD40L-stromal cell conditions for 96 h. Two-tailed unpaired t-test between two groups within a cell line (mean ± s.e.m., n = 3, where each dot is a hydrogel-based organoid). c, Ratio of MFI of pNF-κB/NF-κB in human ABC-DLBCL PDX cells cultured in ±CD40L-stromal cell conditions for 96 h. Two-tailed unpaired t-test (mean ± s.e.m., n = 5 for PDX#2). d, CD40 (green) spatial localization relative to IgM BCR (magenta) puncta in HBL1 ABC-DLBCLs grown in REDV-functionalized hydrogels in the presence of CD40L-stromal cells. Left: 3D projection of IgM BCR, DAPI (blue), and CD40. Right: Orthogonal projection with IgM BCR, DAPI, and actin (orange).Data representative of n = 3 organoids. e, 3D projection of spatial expression of TRAF3 (green) in HBL1 ABC-DLBCLs grown in REDV-functionalized hydrogels in the presence or absence of CD40L-stromal cells, with DAPI (blue) and actin (magenta). Data representative of n = 3 organoids.
