Extended Data Fig. 1: Pre-treatment of fibroblasts with inhibitors of actomyosin contractility further showed the key role of microtubules in the formation of cellular protrusions. | Nature Materials

Extended Data Fig. 1: Pre-treatment of fibroblasts with inhibitors of actomyosin contractility further showed the key role of microtubules in the formation of cellular protrusions.

From: Tension anisotropy drives fibroblast phenotypic transition by self-reinforcing cell–extracellular matrix mechanical feedback

Extended Data Fig. 1

Pre-treatment of cells with actomyosin inhibitors (cytochalasin D, blebbistatin, and Y-27632) caused cells to develop more and longer protrusions. These protrusions exhibited accumulations of tubulin signals, indicative of a microtubule-rich core. Experiments were conducted with three independent biological replicates, all of which yielded similar results. Scale bar: 10 μm.

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