Extended Data Fig. 7: P-KN93 inhibits intracellular signal transduction induced by BTO/rGO + US treatment.
(a) Schematic of intracellular signal transduction after P-KN93 treatment. (b) Western blot data derived from three independent experiments and (c) associated analyses of Tuj1 and MAP2 levels in NSCs treated with or without P-KN93. (d) Western blot data derived from three independent experiments and (e) associated analysis of P-CREB, CREB and BDNF levels in NSCs treated with or without P-KN93. (f) Immunofluorescence staining for P-CREB in NSCs treated with or without P-KN93 (red: F-actin; green: P-CREB; blue: DAPI). (g) Statistical analysis of the fluorescence intensity of P-CREB. (h) Immunofluorescence staining for BDNF in NSCs treated with or without P-KN93 (red: F-actin; green: BDNF; blue: DAPI). (i) Statistical analysis of the fluorescence intensity of BDNF. (j) Measurement of the amount of BDNF secreted by NSCs treated with or without P-KN93 by ELISA. The data in c, e, g, i and j are shown as mean ± s.d., n = 3 independent biological samples. Statistical analyses were performed via one-way ANOVA with Dunnett’s multiple comparisons tests (NS: not significant, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001). Figure elements in a have been created with Figdraw.com.
