Extended Data Fig. 2: Influence of BTO nanoparticles on NSCs and PC12 cells under ultrasound irradiation.
(a–b) mRNA levels of Tuj1 and MAP2 in NSCs after 5 days. (c) Western blot of Tuj1 and MAP2 levels in NSCs after 5 days and (d) associated analyses. (e–f) mRNA levels of Tuj1 and MAP2 in PC12 cells after 5 days. (g) Western blot of Tuj1 and MAP2 levels in PC12 cells after 5 days and (h) associated analyses. (i) Images from the live/dead assay (green: live cell; red: dead cell) and (j) analysis of the survival rate of NSCs after 3 days. (k) Images from the live/dead assay (green: live cell; red: dead cell) and (l) analysis of the survival rate of PC12 cells after 3 days. (m) CCK-8 assay results for NSCs. (n) CCK-8 assay results for PC12 cells. (o) EdU proliferation assay results for NSCs (green: proliferating cells; blue: Hoechst). (p) The proliferation ability of NSCs was assessed via the EdU proliferation assay. (q) EdU proliferation assay results for PC12 cells (green: proliferating cells; blue: Hoechst). (r) The proliferation ability of PC12 cells was assessed via the EdU proliferation assay. The data in a, b, d-f, h, j, l-n, p and r are shown as mean ± s.d., n = 3 independent biological samples for a, b, d-f, h, j, l, p and r; n = 5 independent biological samples for the data in m and n. Statistical analyses were performed via one-way ANOVA with Dunnett’s multiple comparisons tests (NS: not significant, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001).
