Extended Data Fig. 7: Integrin and focal adhesion formation on DECIPHER scaffolds. | Nature Materials

Extended Data Fig. 7: Integrin and focal adhesion formation on DECIPHER scaffolds.

From: Hybrid hydrogel–extracellular matrix scaffolds identify biochemical and mechanical signatures of cardiac ageing

Extended Data Fig. 7: Integrin and focal adhesion formation on DECIPHER scaffolds.

ICC of F-actin, nucleus, α5 integrin, and paxillin in young CFs (a, c) and aged CFs (b, d) on DECIPHER samples (SoftY, StiffY, SoftA, or StiffA). α5 integrin insets are indicated by the black boxes, paxillin insets are indicated by the white boxes, and white arrows point to focal adhesions. The number and area of focal adhesion sites in young CFs (e, f) and aged CFs (g, h) quantified from paxillin staining. N = 3 biological replicates for each condition (e-h). Data are mean \(\pm \,\)SD (e, g). One-way ANOVA with Fisher’s preplanned multiple comparison (each pair with one variable, ECM or stiffness, e-h). Scale bar: 50 µm.

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