Extended Data Fig. 3: Additional CF gene expression levels quantified by RNA-seq.

Normalized gene expression on different DECIPHER samples (SoftY, StiffY, SoftA, StiffA). Quantified RNA-seq data for genes regulating young or aged CF activation (a), mechanosensing (b, c, d, e), ECM remodeling (f, g, h, i, j, k, l), and senescence (m). Specificity analysis of primary CF isolation reveals a pure vimentin-expressing (Vim) CF population with negligible CD31-expressing (Pecam1) endothelial cells and troponin-I-expressing (Tnni3) cardiomyocytes (n). N = 3 biological replicates for each condition, except Young CF+SoftY group in RNA-seq (N = 2). Data are mean \(\pm \,\)SEM (a-n). One-way ANOVA with Fisher’s preplanned multiple comparison (each pair with one variable, ECM or stiffness, a-n).