Extended Data Fig. 7: Resolution of TRACE printed filaments using 2 mg/mL neutralized collagen bioink.
From: Instant assembly of collagen for tissue engineering and bioprinting
(a) Representative images of single filament printed in PEG only bath (brightfield microscopy), and filament with bioink mixed with fluorescent beads printed in PEG+agarose granular support bath (confocal microscopy). Filament diameter was tuned by varying the combination of flow rate Q and printing speed v. Scale bars, 100 μm. (b) Filament diameter d was measured at 16 distinct combinations of flow rate Q and velocity v, from lowest to highest, with three filaments examined per combination. The results are shown as mean ± s.d., with sample sizes of n = 22, 15, 11, 11, 12, 12, 12, 12, 10, 10, 10, 10, 10, 10, 10, and 10 for each combination, respectively. The exact Q and v values used are listed in the source data. A strong agreement is observed between d and \(2\sqrt{Q/\pi v}\), highlighting the effectiveness of the conservation model in predicting TRACE printed collagen filament dimensions. The line in the plot represents \(d=2\sqrt{Q/\pi v}\).
