Extended Data Fig. 3: Macroscopic liver cell constructs.
From: Instant assembly of collagen for tissue engineering and bioprinting
(a) Macrograph and (b) schematic showing the fabricating procedure of the parallel channel device for rapid strip production. (c) Schematic of fabricating cell-lade macroscopic strips. (d) Viability assay on HepG2 strips after fabrication (Day 0) and after 24-hour culture. Scale bars, 200 μm. (e) Histological staining of PAS and PAS with diastase (PAS/D) on a fixed 8-day HepG2 strip. Scale bars, 200 μm. (f) A HepG2 strip (1 × 107 cell/mL) cultured freely in the medium for 8 days. Scale bar, 1 mm. In the ROIs, actin and nucleus were stained. Scale bars, 200 μm. (g) Assembling the Day-7 HepG2 strips into a macroscopic complex pattern “YALE” in a culture medium-rich granular support. Scale bar, 2 mm.
