Extended Data Fig. 3: Cryo-EM micrographs of early stages of drying-induced assembly.
From: Adaptive peptide dispersions enable drying-induced biomolecule encapsulation
a. Drop of 2 µl WYK solution was applied onto the TEM grid and went through evaporation under ambient conditions for indicated time and proceeded with cryo-EM. Cryo-EM images illustrate the structural transformations from random aggregates and short fibers to spherical assemblies. Scale bar = 500 nm; b. Cryo-EM micrographs of different resolutions captured at 5’03” show the process of evaporation-driven porous droplet formation, and the temporal information could be decoded from spatial information; c. Different stages of the assembly process are captured. 1. early stage formation of nanosized spherical assembly; 2-4. nano-sized air pockets inside spherical peptide assemblies; 5-7. larger spheres with multiple pores; 8. fusion of larger spheres with multiple pores. Scale bar = 200 nm.
